Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system.
Identifieur interne : 004802 ( Main/Exploration ); précédent : 004801; suivant : 004803Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system.
Auteurs : Shixia Wang [États-Unis] ; Te-Hui W. Chou ; Pavlo V. Sakhatskyy ; Song Huang ; John M. Lawrence ; Hong Cao ; Xiaoyun Huang ; Shan LuSource :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Cellules Vero, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Immunisation, Lapins, Lignée cellulaire, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Tests de neutralisation, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Vaccins à ADN (administration et posologie), Vaccins à ADN (immunologie), Virus du SRAS (immunologie), Épitopes (), Épitopes (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux, Vaccins à ADN.
- immunologie : Anticorps antiviraux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS, Épitopes.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- Animaux, Cellules Vero, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Immunisation, Lapins, Lignée cellulaire, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Tests de neutralisation, Épitopes.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Epitopes (chemistry), Epitopes (immunology), Humans, Immunization, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Neutralization Tests, Rabbits, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA, Viral Vaccines.
- chemical , chemistry : Epitopes, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Epitopes, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Humans, Immunization, Neutralization Tests, Rabbits, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).
DOI: 10.1128/JVI.79.3.1906-1910.2005
PubMed: 15650214
Affiliations:
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Le document en format XML
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Chou</name></author><author><name sortKey="Sakhatskyy, Pavlo V" sort="Sakhatskyy, Pavlo V" uniqKey="Sakhatskyy P" first="Pavlo V" last="Sakhatskyy">Pavlo V. Sakhatskyy</name></author><author><name sortKey="Huang, Song" sort="Huang, Song" uniqKey="Huang S" first="Song" last="Huang">Song Huang</name></author><author><name sortKey="Lawrence, John M" sort="Lawrence, John M" uniqKey="Lawrence J" first="John M" last="Lawrence">John M. Lawrence</name></author><author><name sortKey="Cao, Hong" sort="Cao, Hong" uniqKey="Cao H" first="Hong" last="Cao">Hong Cao</name></author><author><name sortKey="Huang, Xiaoyun" sort="Huang, Xiaoyun" uniqKey="Huang X" first="Xiaoyun" last="Huang">Xiaoyun Huang</name></author><author><name sortKey="Lu, Shan" sort="Lu, Shan" uniqKey="Lu S" first="Shan" last="Lu">Shan Lu</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Epitopes (chemistry)</term><term>Epitopes (immunology)</term><term>Humans</term><term>Immunization</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Neutralization Tests</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (immunology)</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Immunisation</term><term>Lapins</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (immunologie)</term><term>Épitopes ()</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Epitopes</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Epitopes</term><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Humans</term><term>Immunization</term><term>Neutralization Tests</term><term>Rabbits</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Immunisation</term><term>Lapins</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Cao, Hong" sort="Cao, Hong" uniqKey="Cao H" first="Hong" last="Cao">Hong Cao</name><name sortKey="Chou, Te Hui W" sort="Chou, Te Hui W" uniqKey="Chou T" first="Te-Hui W" last="Chou">Te-Hui W. Chou</name><name sortKey="Huang, Song" sort="Huang, Song" uniqKey="Huang S" first="Song" last="Huang">Song Huang</name><name sortKey="Huang, Xiaoyun" sort="Huang, Xiaoyun" uniqKey="Huang X" first="Xiaoyun" last="Huang">Xiaoyun Huang</name><name sortKey="Lawrence, John M" sort="Lawrence, John M" uniqKey="Lawrence J" first="John M" last="Lawrence">John M. Lawrence</name><name sortKey="Lu, Shan" sort="Lu, Shan" uniqKey="Lu S" first="Shan" last="Lu">Shan Lu</name><name sortKey="Sakhatskyy, Pavlo V" sort="Sakhatskyy, Pavlo V" uniqKey="Sakhatskyy P" first="Pavlo V" last="Sakhatskyy">Pavlo V. Sakhatskyy</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Wang, Shixia" sort="Wang, Shixia" uniqKey="Wang S" first="Shixia" last="Wang">Shixia Wang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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